Abstract Aiming to provide a new way that preserves more active components in Eucommia ulmoides (EU) leaves, this paper utilizes 4 methods, i.e., microwave, steam, air heating, and frying, to process EU leaves before drying, and their respective effects on the leaves were evaluated. The antioxidant activity of EU leaves in vitro was measured by the DPPH free radical method; the contents of total flavonoids (TFC) and total phenols (TPC) were analyzed by spectrophotometry; and the contents of main active components were determined by high-performance liquid chromatography (HPLC). The results showed the pre-process improved the quality of the EU leaves. As a simple and fast process, the microwave process is the most effective technique in the retention of flavonoids and four active ingredients except aucubin. High power (800 W) and short time (2.5 min) are more conducive to the retention of active ingredients, with high antioxidant activity. Appropriate steam and frying processes also exhibited benefits for the active components. In particular, the samples treated with steam for 10 minutes showed great potential for industrial application with the highest antioxidant activity and polyphenol content. Therefore, proper pre-processing before drying should be conducted to effectively protect the active components of EU leaves.

ABSTRACT Introduction: Recent research on the probability of increasing physical injuries during physical activities revealed that resistance training can improve physical performance of college students, prevent sports injuries and reduce the body fat rate of its practitioners. Objective: Analyze the effects of resistance training on the body index of university students. Methods: One hundred female college students were selected as experimenters to ensure normal activities for 16 weeks. The experimenter performed resistance training for 16 weeks, three times a week on Monday, Wednesday and Friday, with three cycles each time. Results: After 16 weeks of experimental intervention, the average chest circumference, average waist circumference, average hip circumference, and average leg circumference of the experimental group were 83.27cm, 63.1cm, 89.95cm, 54.6cm, and 24.02%, respectively. After the experiment, the average back muscle strength of the experimental group increased by 5.11kg, and the average basal metabolism increased from 1204.4 kcal to 1260.59 kcal. Conclusion: Resistance training and aerobic exercise have the most significant effect on body fat rate. Resistance training can control the decline of body fat rate in college students to improve their physical quality. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción: Recientes investigaciones sobre la preocupación por la probabilidad de aumento de lesiones físicas durante las actividades físicas revelaron que el entrenamiento de resistencia puede mejorar el rendimiento físico de los estudiantes universitarios, prevenir lesiones deportivas y reducir el índice de grasa corporal de sus practicantes. Objetivo: Analizar los efectos del entrenamiento de resistencia física sobre el índice corporal de estudiantes universitarios. Métodos: Se seleccionaron cien estudiantes universitarias como experimentadoras para garantizar una actividad normal durante 16 semanas. El experimentador realizó un entrenamiento de resistencia durante 16 semanas, tres veces por semana, los lunes, miércoles y viernes, con tres ciclos cada vez. Resultados: Tras 16 semanas de intervención experimental, el perímetro torácico medio, el perímetro medio de la cintura, el perímetro medio de la cadera y el perímetro medio de las piernas del grupo experimental fueron de 83,27 cm, 63,1 cm, 89,95 cm, 54,6 cm y 24,02% respectivamente. Tras el experimento, la fuerza muscular media de la espalda del grupo experimental aumentó en 5,11 kg, y el metabolismo basal medio pasó de 1204,4 kcal a 1260,59 kcal. Conclusión: El entrenamiento de resistencia y el ejercicio aeróbico tienen el efecto más significativo sobre el índice de grasa corporal. El entrenamiento de resistencia puede controlar la disminución de la tasa de grasa corporal en los estudiantes universitarios, con el fin de mejorar su calidad física. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

RESUMO Introdução: Pesquisas recentes sobre a preocupação na probabilidade do aumento em lesões físicas durante as atividades físicas revelaram que o treinamento de resistência pode melhorar o desempenho físico dos estudantes universitários, prevenir lesões esportivas e reduzir a taxa de gordura corporal de seus praticantes. Objetivo: Analisar os efeitos do treinamento de resistência física sobre o índice corporal dos estudantes universitários. Métodos: Cem estudantes universitárias foram selecionadas como experimentadoras para garantir atividades normais durante 16 semanas. O experimentador realizou treinamento de resistência durante 16 semanas, três vezes por semana na segunda, quarta e sexta-feira, com três ciclos de cada vez. Resultados: Após 16 semanas de intervenção experimental, a circunferência média do tórax, circunferência média da cintura, circunferência média do quadril e circunferência média das pernas do grupo experimental foram 83,27cm, 63,1cm, 89,95cm, 54,6cm e 24,02% respectivamente. Após o experimento, a força média dos músculos das costas do grupo experimental aumentou em 5,11kg, e o metabolismo basal médio aumentou de 1204,4kcal para 1260,59kcal. Conclusão: O treinamento de resistência e o exercício aeróbico têm o efeito mais significativo na taxa de gordura corporal. O treinamento de resistência pode controlar o declínio da taxa de gordura corporal dos estudantes universitários, de forma a melhorar sua qualidade física. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

Abstract The contents of total phenol and flavonoid of 27 edible plants as well as their antioxidative and digestive enzymes inhibitory activities were evaluated. The total phenol content of the 27 plants ranged from 1.28-46.80 mg gallic acid equivalent/g. DPPH, ABTS and reducing power assay explored that Rubus idaeus Linn, Hibiscus sabdariffa Linn. and Crataegus pinnatifida Bge. exhibited stronger antioxidant capacity among the 27 plants. 4 plants showed inhibitory percentage more than 50% on pancreatic lipase. 10 plants showed inhibitory percentage more than 50% on α-glucosidase. 3 plants simultaneously possess significant inhibitory activities on pancreatic lipase and α-glucosidase, which were Alpinia officinarum Hance, Ginkgo biloba Linn. leaves and Vitis vinifera Linn. Seeds. Strong positive correlation between total phenol content and antioxidative capacity were found. However, digestive enzymes inhibitory activity was less correlated with total phenol and total flavonoids content.

Abstract The laminated beam with uniformly vertical displacement can be divided into contact region and separation region when bending deformation occurs. Friction between the interfaces will cause the stiffness inconsistency between the separation region and contact region. In this paper, calculation formula of section stiffness considering interface friction effect is derived. Assuming that the beam vibrates freely in equal wavelength and equal stiffness forms respectively, the calculating formulas of natural vibration frequencies of simply supported and cantilever beams are derived. Finally, based on a steel-concrete laminated test beam with uniform vertical displacement, the natural vibration frequencies of the beam are calculated. The conclusions are as follows: The derived formulas can calculate the natural vibration frequencies of laminated beams under different interface states effectively; The influence of friction effect on the vibration frequency of laminated beams becomes more and more obvious with the increase of the order.

RESUMO A saúde nacional é uma nova exigência para o desenvolvimento social na nova era e na situação atual. Atualmente, existe um problema de desenvolvimento desequilibrado do sistema de serviço público de desporto entre várias regiões da China, com sobretudo uma grande lacuna entre as regiões leste e centro-oeste. A fim de compreender o estado de desenvolvimento do sistema de serviço público de desportos nas regiões central e ocidental a partir da Perspectiva da estratégia nacional de saúde, este estudo construiu o sistema de índice de avaliação do serviço público de desporto nas regiões central e ocidental, e revisou os indicadores através de duas rondas de inquéritos especializados, e determinou o peso do índice por meio do o processo hierárquico analítico (Analytic Hierarchy Process — AHP). Nesta base, verificou-se a validade e a racionalidade do modelo de avaliação, tomando uma cidade na região central e ocidental como representativa do desenvolvimento desportivo público. A pesquisa mostra que o sistema público de serviço esportivo da Cidade tem um bom nível e o sistema de serviço desportivo é relativamente perfeito, o que pode atender às necessidades diversificadas dos residentes que praticam esportes, em certa medida. Por conseguinte, o impulso para o desenvolvimento do sistema público de serviços desportivos nas regiões central e ocidental é positivo, mas ainda precisa de promover a alocação racional dos recursos desportivos regionais. Espera-se que este estudo possa fornecer certa referência para o desenvolvimento do sistema de serviço público de desporto nas regiões centrais e ocidentais, a partir da Perspectiva da estratégia nacional de saúde.

ABSTRACT National health is a new requirement for social development in the new era and in the current situation. At present, there is a problem of unbalanced development of public sports service system among various regions in China, especially the large gap between the East and the Midwest. In order to understand the development status of public sports service system in central and western regions from the perspective of national health strategy, this study constructed the evaluation index system of public sports service in central and western regions, and revised the indicators through two rounds of expert survey, and determined the index weight using AHP. On this basis, taking a city in the central and western region to represent public sports development, the validity and rationality of the evaluation model are verified. The research shows that the public sports service system of the city has a good level and the sports service system is relatively perfect, which can meet the diversified needs of residents participating in sports to a certain extent. Therefore, the development momentum of public sports service system in central and western regions is good, but it still needs to further promote the rational allocation of regional sports resources. It is hoped that this study can provide certain reference for the development of public sports service system in central and western regions from the perspective of national health strategy.

RESUMEN Resumen: La salud nacional es un nuevo requisito para el desarrollo social en la nueva era y en la situación actual. En la actualidad, existe un problema de desarrollo desequilibrado del sistema de servicios deportivos públicos entre varias regiones de China, especialmente la gran brecha entre el Este y el Medio Oeste. Con el fin de comprender el estado de desarrollo del sistema de servicios deportivos públicos en las regiones central y occidental desde la perspectiva de la estrategia nacional de salud, este estudio construyó el sistema de índices de evaluación del servicio deportivo público en las regiones central y occidental. Con ese sistema revisó los indicadores a través de dos rondas de encuestas entre expertos y determinó el peso del índice utilizando el proceso de jerarquía analítica (PJA). Sobre esta base, tomando una ciudad de la región central y una de la occidental para representar el desarrollo deportivo público, se verificó la vigencia y racionalidad del modelo de evaluación. La investigación muestra que el sistema público de servicios deportivos de la ciudad tiene un buen nivel y el sistema de servicios deportivos es relativamente perfecto, lo que puede satisfacer las diversas necesidades de los residentes que participan en deportes. Por lo tanto, el desarrollo del sistema de servicios deportivos públicos en las regiones central y occidental es bueno, pero aún debe promover más la asignación racional de los recursos deportivos regionales. Se espera que este estudio pueda proporcionar una referencia para el desarrollo del sistema público de servicios deportivos en las regiones central y occidental desde la perspectiva de la estrategia nacional de salud.

In this paper, a new notion of S* closedness in L-topological Spaces is introduced by means of semi-open L-"sets and their inequality where L is a complete DeMorgan algebra.This new definition doesn´ t rely on the structure of basic lattice L. It can be characterized by means of semi-open L-"sets and their inequality . When L is completely distributive DeMorgan algebra, its many characterizations are presented.

Objective: To investigate the functions of Fibroblast Growth Factor Receptor-2 (FGFR2) at different stages of cell differentiation. The engineered murine embryonic stem (ES) cells with conditional knockout of FGFR2 were developed depending on Cre-loxP. Methods: Cre-loxP system was used in a conditional targeting vector. The competent AM-1 bacteria, which expressed Cre-recombinase, was used to confirm the Cre-mediated deletion of the floxed exons 7 and 8 of FGFR2. The targeting vector was electroporated into the ES cells, and the transfected ES cells were screened with G418 and Ganciclovir. Finally, the ES clones with correct targeting events were identified by Southern Blot and PCR. Results: The targeting vector with conditional knockout of murine FGFR2 was successfully constructed and confirmed by PCR and digestion analysis in bacteria. 86 ES clones were collected by selective culture with G418 and Ganciclovir. Four of the 86 ES clones were found containing the targeting gene sequence in genomic DNA proved by Southern Blot with a 5'-end flank probe. Two of the four ES clones had the correct targeting events that included the insertion of the targeting gene sequence in genomic DNA and were checked by Southern Blot with a 3'-end flanking probe. Finally, the insertion of loxP (loxP3) between exons 8 and 9 in genomic DNA was identified in one of the two ES clones by Southern Blot and PCR. Conclusion: FGFR2 conditional knockout depending on Cre-loxP can be successfully used in ES cells.